A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H, Yanagida M

BACKGROUND: Anaphase-promoting complex (APC)/cyclosome and 26S proteasome are respectively required for polyubiquitination and degradation of mitotic cyclin and anaphase inhibitor Cut2 (Pds1/securin). In fission yeast, mutant cells defective in cyclosome and proteasome fail to complete mitosis and have hypercondensed chromosomes and a short spindle. A similar phenotype is seen in a temperature-sensitive strain cut8-563 at 36 degrees C, but the ... [more]

Curr. Biol. Nov. 02, 2000; 10(21);1329-38 [Pubmed: 11084332]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CUT8 RPN12	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Takeda K (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CUT8

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]protein anchor [TAS]sterol binding [IDA]

Gene Ontology Cellular Component

RPN12