Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Substrate-Trapped Interactors of PHD3 and FIH Cluster in Distinct Signaling Pathways.

Rodriguez J, Pilkington R, Garcia Munoz A, Nguyen LK, Rauch N, Kennedy S, Monsefi N, Herrero A, Taylor CT, von Kriegsheim A

Amino acid hydroxylation is a post-translational modification that regulates intra- and inter-molecular protein-protein interactions. The modifications are regulated by a family of 2-oxoglutarate- (2OG) dependent enzymes and, although the biochemistry is well understood, until now only a few substrates have been described for these enzymes. Using quantitative interaction proteomics, we screened for substrates of the proline hydroxylase PHD3 and the ... [more]

Cell Rep Mar. 22, 2016; 14(11);2745-60 [Pubmed: 26972000]

Throughput

  • High Throughput

Additional Notes

  • Source of EGLN3 not clear

Curated By

  • BioGRID