An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation.

Lampert F, Stafa D, Goga A, Soste MV, Gilberto S, Olieric N, Picotti P, Stoffel M, Peter M

In yeast, the glucose-induced degradation-deficient (GID) E3 ligase selectively degrades superfluous gluconeogenic enzymes. Here, we identified all subunits of the mammalian GID/CTLH complex and provide a comprehensive map of its hierarchical organization and step-wise assembly. Biochemical reconstitution demonstrates that the mammalian complex possesses inherent E3 ubiquitin ligase activity, using Ube2H as its cognate E2. Deletions of multiple GID subunits compromise ... [more]

Elife Jun. 18, 2018; 7(); [Pubmed: 29911972]

Throughput

Low Throughput

Additional Notes

ubiquitination activity: E1 is Ube1, E2 (bait) is Ube2H, E3/accessory factors are RanBP9, Mkln1, WDR26, ARMC8, RanBP10, RMND5a, GID4, Twa1, YPEL5; substrate is Hbp1 (hit)

Curated By

BioGRID

Interaction Summary

UBE2H

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]small conjugating protein transferase activity [IBA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

HBP1