An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Substrate-Trapped Interactors of PHD3 and FIH Cluster in Distinct Signaling Pathways.

Rodriguez J, Pilkington R, Garcia Munoz A, Nguyen LK, Rauch N, Kennedy S, Monsefi N, Herrero A, Taylor CT, von Kriegsheim A

Amino acid hydroxylation is a post-translational modification that regulates intra- and inter-molecular protein-protein interactions. The modifications are regulated by a family of 2-oxoglutarate- (2OG) dependent enzymes and, although the biochemistry is well understood, until now only a few substrates have been described for these enzymes. Using quantitative interaction proteomics, we screened for substrates of the proline hydroxylase PHD3 and the ... [more]

Cell Rep Mar. 22, 2016; 14(11);2745-60 [Pubmed: 26972000]

Throughput

High Throughput

Additional Notes

Source of MAPK6 not clear

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAPK6 BAG6	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vinayagam A (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MAPK6

Gene Ontology Biological Process

Gene Ontology Molecular Function

MAP kinase activity [ISS]protein binding [IPI]protein serine/threonine kinase activity [TAS]

Gene Ontology Cellular Component

BAG6

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [ISS]proteasome binding [ISS]protein binding [IPI]ribosome binding [IDA]ubiquitin protein ligase binding [IPI]