SPOP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GLI2
Gene Ontology Biological Process
- axon guidance [ISS]
- branching morphogenesis of an epithelial tube [ISS]
- cell proliferation [IDA]
- cellular response to virus [IDA]
- cerebellar cortex morphogenesis [ISS]
- developmental growth [ISS]
- embryonic digestive tract development [ISS]
- epidermal cell differentiation [IDA]
- floor plate formation [ISS]
- heart development [ISS]
- hindbrain development [ISS]
- hindgut morphogenesis [ISS]
- kidney development [ISS]
- lung development [ISS]
- mammary gland development [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- neuron development [ISS]
- odontogenesis of dentin-containing tooth [ISS]
- osteoblast development [ISS]
- osteoblast differentiation [IDA]
- pattern specification process [ISS]
- pituitary gland development [ISS]
- positive regulation of DNA replication [IDA]
- positive regulation of T cell differentiation in thymus [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- proximal/distal pattern formation [ISS]
- skeletal system development [ISS]
- smoothened signaling pathway [IDA]
- smoothened signaling pathway involved in ventral spinal cord interneuron specification [ISS]
- spinal cord dorsal/ventral patterning [ISS]
- spinal cord ventral commissure morphogenesis [ISS]
- tube development [ISS]
- ventral midline development [ISS]
- ventral spinal cord development [ISS]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.
Bromodomain and extraterminal domain (BET) protein inhibitors are emerging as promising anticancer therapies. The gene encoding the E3 ubiquitin ligase substrate-binding adaptor speckle-type POZ protein (SPOP) is the most frequently mutated in primary prostate cancer. Here we demonstrate that wild-type SPOP binds to and induces ubiquitination and proteasomal degradation of BET proteins (BRD2, BRD3 and BRD4) by recognizing a degron ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GLI2 SPOP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3320128 | |
| GLI2 SPOP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SPOP GLI2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GLI2 SPOP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID