BAIT

PTK2

FAK, FRNK, Fadk, mKIAA4203, p125FAK
PTK2 protein tyrosine kinase 2
GO Process (50)
GO Function (12)
GO Component (14)

Gene Ontology Biological Process

Mus musculus
PREY

SRC

AW259666, pp60c-src, RP23-169M4.1
Rous sarcoma oncogene
GO Process (29)
GO Function (9)
GO Component (6)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Contribution of Src and Ras pathways in FGF-2 induced endothelial cell differentiation.

Klint P, Kanda S, Kloog Y, Claesson-Welsh L

We have examined fibroblast growth factor (FGF) receptor-1 mediated signal transduction in differentiation of endothelial cells (EC). The activated FGFR-1 couples to Ras through two adaptor proteins, FRS2 and Shc. In FGF-2 treated proliferating EC, FRS2 as well as Shc are tyrosine phosphorylated and interact with Grb2. In contrast, in FGF-2 treated differentiating cells, Shc, but not FRS2, is engaged ... [more]

Oncogene Jun. 03, 1999; 18(22);3354-64 [Pubmed: 10362356]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PTK2 SRC
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1533936
SRC PTK2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9415BioGRID
2673435

Curated By

  • BioGRID