An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates.

Nguyen TV, Li J, Lu CJ, Mamrosh JL, Lu G, Cathers BE, Deshaies RJ

Glutamine synthetase (GS) plays an essential role in metabolism by catalyzing the synthesis of glutamine from glutamate and ammonia. Our recent study showed that CRBN, a direct protein target for the teratogenic and antitumor activities of immunomodulatory drugs such as thalidomide, lenalidomide, and pomalidomide, recognizes an acetyl degron of GS, resulting in ubiquitylation and degradation of GS in response to ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 04, 2016; 114(14);3565-3571 [Pubmed: 28320958]

Throughput

Low Throughput

Additional Notes

Source of NPLOC4 not clear

Curated By

BioGRID

Interaction Summary

NPLOC4

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

GLUL