An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain.

Valnegri P, Huang J, Yamada T, Yang Y, Mejia LA, Cho HY, Oldenborg A, Bonni A

Although ubiquitin ligases have been implicated in autism, their roles and mechanisms in brain development remain incompletely understood. Here, we report that in vivo knockdown or conditional knockout of the autism-linked ubiquitin ligase RNF8 or associated ubiquitin-conjugating enzyme UBC13 in rodent cerebellar granule neurons robustly increases the number of parallel fiber presynaptic boutons and functional parallel fiber/Purkinje cell synapses. In ... [more]

Nat Commun Dec. 02, 2016; 8(1);1271 [Pubmed: 29097665]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RNF8

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [ISO, ISS]histone binding [ISO, ISS]protein homodimerization activity [ISO, ISS]ubiquitin protein ligase activity [ISO]ubiquitin protein ligase binding [ISO]ubiquitin-protein transferase activity [ISO, ISS]zinc ion binding [ISO, ISS]

Gene Ontology Cellular Component

KTN1