An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Molecular determinants of KA1 domain-mediated autoinhibition and phospholipid activation of MARK1 kinase.

Emptage RP, Lemmon MA, Ferguson KM

Protein kinases are frequently regulated by intramolecular autoinhibitory interactions between protein modules that are reversed when these modules bind other 'activating' protein or membrane-bound targets. One group of kinases, the MAP/microtubule affinity-regulating kinases (MARKs) contain a poorly understood regulatory module, the KA1 (kinase associated-1) domain, at their C-terminus. KA1 domains from MARK1 and several related kinases from yeast to humans ... [more]

Biochem. J. Dec. 01, 2016; 474(3);385-398 [Pubmed: 27879374]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MARK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]magnesium ion binding [IDA]phosphatidic acid binding [IDA]phosphatidylinositol-4,5-bisphosphate binding [IDA]phosphatidylserine binding [IDA]protein serine/threonine kinase activity [IDA]tau-protein kinase activity [ISS]

Gene Ontology Cellular Component