BAIT

APC15

SPBC83.04
anaphase-promoting complex subunit Apc15
Schizosaccharomyces pombe (972h)
PREY

CUT4

apc1, SPBC106.09
anaphase-promoting complex subunit Apc1
GO Process (2)
GO Function (0)
GO Component (3)
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.

Yoon HJ, Feoktistova A, Wolfe BA, Jennings JL, Link AJ, Gould KL

The anaphase-promoting complex (APC) is a conserved multisubunit ubiquitin ligase required for the degradation of key cell cycle regulators. Components of the APC have been identified through genetic screens in both Schizosaccharomyces pombe and Saccharomyces cerevisiae as well as through biochemical purification coupled with mass spectrometric protein identification. With these approaches, 11 subunits of the core S. cerevisiae APC have ... [more]

Curr. Biol. Dec. 10, 2002; 12(23);2048-54 [Pubmed: 12477395]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUT4 APC15
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID