BAIT
PMA1
KTI10, H(+)-exporting P2-type ATPase PMA1, L000001449, YGL008C
Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
GO Process (4)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
NUP120
RAT2, L000003138, YKL057C
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
GO Process (13)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- double-strand break repair [IGI, IMP]
- mRNA export from nucleus [IMP]
- mRNA export from nucleus in response to heat stress [IMP]
- maintenance of chromatin silencing at telomere [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- nuclear pore distribution [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IDA, IGI]
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
- protein export from nucleus [IMP]
- protein import into nucleus [IMP]
- ribosomal large subunit export from nucleus [IMP]
- telomere tethering at nuclear periphery [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic identification of genes involved in metabolic acid stress resistance in yeast and their potential as cancer targets.
A hallmark of all primary and metastatic tumours is their high rate of glucose uptake and glycolysis. A consequence of the glycolytic phenotype is the accumulation of metabolic acid; hence, tumour cells experience considerable intracellular acid stress. To compensate, tumour cells upregulate acid pumps, which expel the metabolic acid into the surrounding tumour environment, resulting in alkalization of intracellular pH ... [more]
Dis Model Mech Dec. 01, 2015; 9(9);1039-49 [Pubmed: 27519690]
Throughput
- High Throughput
Ontology Terms
- colony size (APO:0000063)
Additional Notes
- SGA screen performed at pH 4
Curated By
- BioGRID