AHR
Gene Ontology Biological Process
- B cell differentiation [TAS]
- B cell homeostasis [IMP, TAS]
- B-1 B cell homeostasis [TAS]
- T cell homeostasis [IMP]
- blood circulation [IMP]
- blood vessel development [IMP]
- blood vessel morphogenesis [IMP]
- blood vessel remodeling [IMP]
- camera-type eye development [IMP]
- cardiac left ventricle morphogenesis [IMP]
- cell morphogenesis [IMP]
- cellular response to cAMP [IDA]
- circadian regulation of gene expression [IDA]
- circumferential growth involved in left ventricle morphogenesis [IMP]
- common bile duct development [IMP]
- embryonic hemopoiesis [IMP]
- gland development [IMP]
- glomerulus morphogenesis [IMP]
- immune system process [IMP]
- intracellular receptor signaling pathway [IDA, ISO]
- kidney morphogenesis [IMP]
- liver development [IMP]
- lymphocyte homeostasis [IMP, TAS]
- negative regulation of necrotic cell death [IMP]
- negative regulation of systemic arterial blood pressure [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of vasoconstriction [IMP]
- ovarian follicle development [ISO]
- patterning of blood vessels [IMP]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- positive regulation of cell size [IMP]
- positive regulation of growth [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- post-embryonic hemopoiesis [IMP]
- prostate gland development [IMP]
- regulation of B cell proliferation [ISO]
- regulation of blood pressure [TAS]
- regulation of blood vessel size [IMP]
- regulation of gene expression [ISO]
- regulation of heart growth [TAS]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA, ISO, TAS]
- reproductive structure development [IMP]
- response to organic cyclic compound [ISO]
- response to stress [IDA]
- response to toxic substance [IDA, ISO]
- response to xenobiotic stimulus [IDA, ISO]
- smooth muscle tissue development [IMP]
- spleen development [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- venous blood vessel development [IMP]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- E-box binding [IDA]
- Hsp90 protein binding [ISO, TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- aryl hydrocarbon receptor activity [ISO]
- enhancer binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO, TAS]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- E-box binding [IDA]
- Hsp90 protein binding [ISO, TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- aryl hydrocarbon receptor activity [ISO]
- enhancer binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO, TAS]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
ARNT
Gene Ontology Biological Process
- cell differentiation [IMP]
- embryonic placenta development [IMP]
- intracellular receptor signaling pathway [IMP]
- positive regulation of hormone biosynthetic process [ISO]
- positive regulation of protein sumoylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation vascular endothelial growth factor production [ISO]
- regulation of nucleic acid-templated transcription [IPI]
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress [ISO]
- regulation of transcription, DNA-templated [TAS]
- response to hypoxia [IDA, ISO]
- response to toxic substance [TAS]
Gene Ontology Molecular Function- DNA binding [IGI]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- aryl hydrocarbon receptor activity [IMP]
- aryl hydrocarbon receptor binding [ISO]
- enhancer binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [IDA]
- transcription factor binding [ISO]
- transcription factor binding transcription factor activity [IPI]
- DNA binding [IGI]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- aryl hydrocarbon receptor activity [IMP]
- aryl hydrocarbon receptor binding [ISO]
- enhancer binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [IDA]
- transcription factor binding [ISO]
- transcription factor binding transcription factor activity [IPI]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Multiple roles of ligand in transforming the dioxin receptor to an active basic helix-loop-helix/PAS transcription factor complex with the nuclear protein Arnt.
The dioxin receptor is a ligand-activated transcription factor belonging to an emerging class of basic helix-loop-helix/PAS proteins which show interaction with the molecular chaperone hsp90 in their latent states and require heterodimerization with a general cofactor, Arnt, to form active DNA binding complexes. Upon binding of polycyclic aromatic hydrocarbons typified by dioxin, the dioxin receptor translocates from the cytoplasm to ... [more]
Throughput
- Low Throughput
Additional Notes
- Electromobility shift assay.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARNT AHR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| AHR ARNT | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARNT AHR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARNT AHR | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2459468 |
Curated By
- BioGRID