BAIT

KCNJ2

Kir2.1
potassium inwardly-rectifying channel, subfamily J, member 2
GO Process (17)
GO Function (4)
GO Component (12)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone.

Utrilla RG, Nieto-Marin P, Alfayate S, Tinaquero D, Matamoros M, Perez-Hernandez M, Sacristan S, Ondo L, de Andres R, Diez-Guerra FJ, Tamargo J, Delpon E, Caballero R

Cardiac Kir2.1 and Nav1.5 channels generate the inward rectifier K+ (IK1) and the Na+ (INa) currents, respectively. There is a mutual interplay between the ventricular INa and IK1 densities, because Nav1.5 and Kir2.1 channels exhibit positive reciprocal modulation. Here we compared some of the biological properties of Nav1.5 and Kir2.1 channels when they are expressed together or separately to get ... [more]

Front Physiol Dec. 01, 2017; 8();903 [Pubmed: 29184507]

Throughput

  • Low Throughput

Curated By

  • BioGRID