BAIT
WDR77
MEP-50, MEP50, Nbla10071, p44, p44/Mep50, HKMT1069
WD repeat domain 77
GO Process (4)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA, IGI]
- cytosol [IDA, TAS]
- methylosome [IDA]
- nucleoplasm [IDA]
- nucleus [IGI]
Homo sapiens
PREY
DDX5
G17P1, HLR1, HUMP68, p68
DEAD (Asp-Glu-Ala-Asp) box helicase 5
GO Process (11)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- cell growth [NAS]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- mRNA splicing, via spliceosome [IC]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- positive regulation of intracellular estrogen receptor signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of alternative mRNA splicing, via spliceosome [IDA]
- regulation of androgen receptor signaling pathway [IMP]
- regulation of osteoblast differentiation [ISS]
- regulation of skeletal muscle cell differentiation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The PRMT5/WDR77 complex regulates alternative splicing through ZNF326 in breast cancer.
We observed overexpression and increased intra-nuclear accumulation of the PRMT5/WDR77 in breast cancer cell lines relative to immortalized breast epithelial cells. Utilizing mass spectrometry and biochemistry approaches we identified the Zn-finger protein ZNF326, as a novel interaction partner and substrate of the nuclear PRMT5/WDR77 complex. ZNF326 is symmetrically dimethylated at arginine 175 (R175) and this modification is lost in a ... [more]
Nucleic Acids Res. Nov. 02, 2017; 45(19);11106-11120 [Pubmed: 28977470]
Throughput
- High Throughput
Curated By
- BioGRID