BTG1
Gene Ontology Biological Process
- cell migration [NAS]
- negative regulation of cell growth [NAS]
- negative regulation of cell proliferation [IDA]
- positive regulation of angiogenesis [IMP]
- positive regulation of endothelial cell differentiation [IMP]
- positive regulation of fibroblast apoptotic process [IMP]
- positive regulation of myoblast differentiation [IDA, NAS]
- regulation of transcription, DNA-templated [NAS]
Gene Ontology Molecular Function
CNOT7
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- carbohydrate metabolic process [TAS]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IDA]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- gene silencing by miRNA [TAS]
- mRNA metabolic process [TAS]
- negative regulation of cell proliferation [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence rec-Y2H protein interactions.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CNOT7 BTG1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | Low | - | BioGRID | 2817776 | |
| BTG1 CNOT7 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| BTG1 CNOT7 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| BTG1 CNOT7 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| BTG1 CNOT7 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID