BAIT

MORF4L1

Eaf3, HsT17725, MEAF3, MORFRG15, MRG15, S863-6, FWP006
mortality factor 4 like 1
Homo sapiens
PREY

SH3GLB2

PP6569, PP9455, RRIG1, RP11-167N5.3
SH3-domain GRB2-like endophilin B2
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.

Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP

Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]

Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]

Throughput

  • High Throughput

Additional Notes

  • High confidence rec-Y2H protein interactions.

Curated By

  • BioGRID