BAIT
PIAS1
DDXBP1, GBP, GU/RH-II, ZMIZ3
protein inhibitor of activated STAT, 1
GO Process (10)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- JAK-STAT cascade [TAS]
- androgen receptor signaling pathway [NAS]
- cytokine-mediated signaling pathway [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of protein sumoylation [IDA]
- positive regulation of transcription, DNA-templated [NAS]
- protein sumoylation [ISS]
- regulation of cell proliferation [ISS]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
TEX11
TGC1, TSGA3, RP11-291O7.2
testis expressed 11
GO Process (2)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]
Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]
Throughput
- High Throughput
Additional Notes
- High confidence rec-Y2H protein interactions.
Curated By
- BioGRID