BAIT
CDKN2D
INK4D, p19, p19-INK4D
cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
GO Process (15)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- DNA synthesis involved in DNA repair [IMP]
- G1/S transition of mitotic cell cycle [IDA]
- autophagic cell death [IMP]
- cell cycle arrest [IDA]
- mitotic cell cycle [TAS]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- negative regulation of phosphorylation [IDA]
- negative regulation of protein serine/threonine kinase activity [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- response to UV [IMP]
- response to retinoic acid [IMP]
- response to vitamin D [IMP]
Gene Ontology Molecular Function
Homo sapiens
PREY
SH3GLB2
PP6569, PP9455, RRIG1, RP11-167N5.3
SH3-domain GRB2-like endophilin B2
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]
Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]
Throughput
- High Throughput
Additional Notes
- High confidence rec-Y2H protein interactions.
Curated By
- BioGRID