Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.

Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP

Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]

Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]

Throughput

  • High Throughput

Additional Notes

  • High confidence rec-Y2H protein interactions.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KLF3 PIAS1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3652087

Curated By

  • BioGRID