BAIT
MYO5A
GS1, MYH12, MYO5, MYR12
myosin VA (heavy chain 12, myoxin)
GO Process (10)
GO Function (3)
GO Component (17)
Gene Ontology Biological Process
- actin filament-based movement [NAS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- membrane organization [TAS]
- post-Golgi vesicle-mediated transport [IMP]
- protein localization to plasma membrane [ISS]
- regulation of Golgi organization [IMP]
- transport [NAS]
- vesicle transport along actin filament [IMP]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [IDA]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [IDA]
- filopodium tip [IDA]
- growth cone [NAS]
- insulin-responsive compartment [ISS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- neuron projection [NAS]
- peroxisome [IDA]
- recycling endosome [IDA]
- ruffle [IDA]
- vesicle [IDA]
Homo sapiens
PREY
NDEL1
EOPA, MITAP1, NDE1L1, NDE2, NUDEL
nudE neurodevelopment protein 1-like 1
GO Process (10)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- cell migration [IBA]
- centrosome localization [IBA]
- chromosome segregation [IMP]
- establishment of chromosome localization [IBA]
- establishment of mitotic spindle orientation [IBA]
- microtubule nucleation [IBA]
- mitotic cell cycle [TAS]
- mitotic centrosome separation [IBA]
- regulation of neuron projection development [IBA]
- vesicle transport along microtubule [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]
Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]
Throughput
- High Throughput
Additional Notes
- High confidence rec-Y2H protein interactions.
Curated By
- BioGRID