SNAP29
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ALPHASNAP
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [NAS]
- compound eye morphogenesis [IMP]
- imaginal disc-derived wing expansion [IMP]
- intra-Golgi vesicle-mediated transport [IMP]
- intracellular protein transport [IMP]
- mitotic cytokinesis [IMP]
- neuromuscular synaptic transmission [IEP]
- neuron projection morphogenesis [IMP]
- neurotransmitter secretion [NAS]
- phagocytosis [IMP]
- positive regulation of SNARE complex disassembly [IDA]
- synaptic transmission [IMP]
- synaptic vesicle fusion to presynaptic membrane [IDA]
- synaptic vesicle priming [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Drosophila SNAP-29 is an essential SNARE that binds multiple proteins involved in membrane traffic.
Each membrane fusion event along the secretory and endocytic pathways requires a specific set of SNAREs to assemble into a 4-helical coiled-coil, the so-called trans-SNARE complex. Although most SNAREs contribute one helix to the trans-SNARE complex, members of the SNAP-25 family contribute two helixes. We report the characterization of the Drosophila homologue of SNAP-29 (dSNAP-29), which is expressed throughout development. ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
SNAP29 ALPHASNAP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
SNAP29 ALPHASNAP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | FlyBase | - | |
SNAP29 ALPHASNAP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |