Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T, Hayashi T, Ikai N, Katayama T, Aoki K, Obara T, Toyoda Y, Maruyama T, Kitagawa D, Takahashi K, Nagao K, Nakaseko Y, Yanagida M

The small genome of fission yeast Schizosaccharomyces pombe contains 4824 predicted genes and gene disruption suggests that approximately 850 are essential for viability. To obtain information on interactions among genes required for chromosome segregation, an approach called Strategy B was taken using mass transformation of the 1015 temperature-sensitive (ts) mutants that were made by random mutagenesis and transformed by plasmids ... [more]

Genes Cells Nov. 01, 2004; 9(11);1069-82 [Pubmed: 15507118]

Throughput

  • Low Throughput

Ontology Terms

  • heat sensitivity (APO:0000147)
  • inviable (APO:0000112)

Curated By

  • BioGRID