A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.

Spiga MG, D'Urso G

DNA polymerase epsilon (Pol epsilon) is a multi-subunit enzyme required for the initiation of chromosomal DNA replication. Here, we report the cloning of two fission yeast genes, called dpb3+ and dpb4+ that encode proteins homologous to the two smallest subunits of Pol epsilon. Although Dpb4 is not required for cell viability, Deltadpb4 mutants are synthetically lethal with mutations in four ... [more]

Nucleic Acids Res. Sep. 25, 2004; 32(16);4945-53 [Pubmed: 15388803]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Curated By

BioGRID

Interaction Summary

DPB4

Gene Ontology Biological Process

Gene Ontology Cellular Component

CDC45

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]protein binding [IPI]