BAIT

CG12404

Dmel\CG12404, Dmel_CG12404
CG12404 gene product from transcript CG12404-RA
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

CG5484

COP, Dmel\CG5484, anon-WO0118547.366, Dmel_CG5484
CG5484 gene product from transcript CG5484-RC
GO Process (3)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Yif1 associates with Yip1 on Golgi and regulates dendrite pruning in sensory neurons during Drosophila metamorphosis.

Wang Q, Wang Y, Yu F

Pruning that selectively removes unnecessary neurites without causing neuronal death is essential for sculpting the mature nervous system during development. In Drosophila, ddaC sensory neurons specifically prune their larval dendrites with intact axons during metamorphosis. However, the important role of endoplasmic reticulum (ER)-to-Golgi transport in dendrite pruning remains unknown. Here, in a clonal screen, we have identified Yif1, an uncharacterized ... [more]

Development Dec. 13, 2017; 145(12); [Pubmed: 29769219]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CG5484 CG12404
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-FlyBase
-
CG12404 CG5484
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By