BAIT
ATB2
alp2, ban5, tub1, SPBC800.05c
tubulin alpha 2
GO Process (3)
GO Function (0)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
BUB1
SPCC1322.12c
mitotic spindle checkpoint kinase Bub1
GO Process (7)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IMP]
- cellular protein localization [IMP]
- histone H2A-S121 phosphorylation [IDA]
- meiotic sister chromatid cohesion, centromeric [IGI]
- mitotic spindle assembly checkpoint [IMP]
- protein localization to kinetochore [IMP]
- signal transduction involved in mitotic spindle assembly checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.
We have identified a novel temperature-sensitive mutant of fission yeast alpha-tubulin Atb2 (atb2-983) that contains a single amino acid substitution (V260I). Atb2-983 is incorporated into the microtubules, and their overall structures are not altered noticeably, but microtubule dynamics is compromised during interphase. atb2-983 displays a high rate of chromosome missegregation and is synthetically lethal with deletions in a subset of ... [more]
Mol. Biol. Cell Mar. 01, 2006; 17(3);1421-35 [Pubmed: 16394105]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID