A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex.

Matsumoto S, Ogino K, Noguchi E, Russell P, Masai H

The protein kinase Hsk1 is essential for DNA replication in Schizosaccharomyces pombe. It associates with Dfp1/Him1 to form an active complex equivalent to the Cdc7-Dbf4 protein kinase in Saccharomyces cerevisiae. Swi1 and Swi3 are subunits of the replication fork protection complex in S. pombe that is homologous to the Tof1-Csm3 complex in S. cerevisiae. The fork protection complex helps to ... [more]

J. Biol. Chem. Dec. 30, 2005; 280(52);42536-42 [Pubmed: 16263721]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SWI1 HSK1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Matsumoto S (2005)	Low	-	BioGRID	-
SWI1 HSK1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Shimmoto M (2009)	Low	-	PomBase	-
SWI1 HSK1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Matsumoto S (2005)	Low	-	BioGRID	248090
HSK1 SWI1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Shimmoto M (2009)	Low	-	PomBase	-
SWI1 HSK1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Dolan WP (2010)	Low	-	BioGRID	442626

Curated By

BioGRID

Interaction Summary

HSK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

SWI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]chromatin binding [IDA]protein binding [IPI]