A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe.

Bottner CA, Schmidt H, Vogel S, Michele M, Kaeufer NF

The spliceosomal component Prp1 (U5-102 kD) is found in Schizosaccharomyces pombe, a physiological substrate of Prp4 kinase. Here, we identify, spp41-1, a previously isolated extragenic suppressor of Prp4 kinase. The gene encodes an ATP-dependent RNA helicase homologous to the splicing factor Brr2 of Saccharomyces cerevisiae and U5-200 kD of mammalia. The suppressor allele, spp41-1, interacts genetically with alleles of prp1. ... [more]

Curr. Genet. Sep. 01, 2005; 48(3);151-61 [Pubmed: 16133344]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRP1 SPP42	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Carnahan RH (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PRP1

Gene Ontology Biological Process

Gene Ontology Cellular Component

SPP42