1-phosphatidylinositol-4-phosphate 5-kinase Its3
GO Process (4)
GO Function (1)
GO Component (2)
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.


Phosphatidylinositol-4-phosphate 5-kinase regulates fission yeast cell integrity through a phospholipase C-mediated protein kinase C-independent pathway.

Deng L, Sugiura R, Ohta K, Tada K, Suzuki M, Hirata M, Nakamura S, Shuntoh H, Kuno T

Fission yeast its3-1 mutant is an allele of the essential gene its3+ that encodes a phosphatidylinositol-4-phosphate 5-kinase (PIP5K) that produces phosphatidylinositol 4,5-bisphosphate. We found that the its3-1 mutant is sensitive to micafungin, a (1,3)-beta-D-glucan synthase inhibitor, suggesting a cell wall integrity defect. Consistently, its3-1 mutation caused synthetic lethality with a (1,3)-beta-D-glucan synthase mutant, bgs1-i2, and its3-1 mutant cells showed aberrant ... [more]

J. Biol. Chem. Jul. 29, 2005; 280(30);27561-8 [Pubmed: 15923187]


  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: heat sensitivity (APO:0000147)

Curated By

  • BioGRID