ECR
Gene Ontology Biological Process
- Malpighian tubule morphogenesis [IMP]
- autophagy [IMP]
- axonogenesis [IMP]
- border follicle cell migration [IMP]
- cardiac muscle tissue development [IMP]
- cardioblast differentiation [IMP]
- cell adhesion [IMP]
- chitin-based embryonic cuticle biosynthetic process [IMP]
- dendrite morphogenesis [IMP, NAS]
- determination of adult lifespan [IMP]
- dorsal vessel heart proper cell fate commitment [IMP]
- ecdysis, chitin-based cuticle [IMP]
- ecdysone receptor-mediated signaling pathway [IMP]
- ecdysone-mediated induction of salivary gland cell autophagic cell death [NAS]
- epidermis development [IMP]
- germ cell development [IMP]
- germ-band shortening [IMP]
- hatching [IMP]
- head involution [IMP]
- histoblast morphogenesis [IMP]
- imaginal disc eversion [TAS]
- imaginal disc-derived wing morphogenesis [IGI, IMP]
- larval central nervous system remodeling [IMP, TAS]
- larval wandering behavior [IMP]
- long-term memory [IMP]
- metamorphosis [IMP]
- muscle organ development [IMP]
- mushroom body development [IGI, IMP]
- negative regulation of growth of symbiont in host [IMP]
- negative regulation of neuroblast proliferation [IMP]
- negative regulation of transcription, DNA-templated [TAS]
- neuron development [IMP]
- neuron remodeling [IMP]
- oogenesis [IMP, NAS]
- peripheral nervous system development [IMP]
- phagocytosis, engulfment [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of circadian sleep/wake cycle, sleep [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription, DNA-templated [IMP]
- pupariation [IMP]
- regulation of Malpighian tubule diameter [IMP]
- regulation of autophagy [IMP]
- regulation of cellular respiration [IMP]
- regulation of development, heterochronic [TAS]
- regulation of hemocyte proliferation [IMP]
- regulation of rhodopsin gene expression [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- response to cocaine [IGI]
- response to ecdysone [IMP]
- salivary gland cell autophagic cell death [IMP]
- sperm individualization [IMP]
- spermatid development [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IPI]
- RNA polymerase II transcription coactivator binding [IPI]
- core promoter binding [IDA]
- ecdysteroid hormone receptor activity [IDA, IPI, NAS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [NAS, TAS]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- repressing transcription factor binding [IPI]
- steroid binding [NAS]
- transcription regulatory region sequence-specific DNA binding [IDA]
- DNA binding [IDA, NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IPI]
- RNA polymerase II transcription coactivator binding [IPI]
- core promoter binding [IDA]
- ecdysteroid hormone receptor activity [IDA, IPI, NAS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [NAS, TAS]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- repressing transcription factor binding [IPI]
- steroid binding [NAS]
- transcription regulatory region sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
TRR
Gene Ontology Biological Process
- compound eye development [IMP, NAS]
- histone H3-K4 methylation [IDA]
- histone methylation [IDA]
- positive regulation of histone H3-K9 trimethylation [IMP]
- positive regulation of transcription, DNA-templated [IGI]
- progression of morphogenetic furrow involved in compound eye morphogenesis [IGI, IMP]
- smoothened signaling pathway [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts.
Nuclear pore complex components (Nups) have been implicated in transcriptional regulation, yet what regulatory steps are controlled by metazoan Nups remains unclear. We identified the presence of multiple Nups at promoters, enhancers, and insulators in the Drosophila genome. In line with this binding, we uncovered a functional role for Nup98 in mediating enhancer-promoter looping at ecdysone-inducible genes. These genes were ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ECR TRR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 197179 | |
| TRR ECR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 197181 | |
| TRR ECR | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| TRR ECR | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 659012 | |
| ECR TRR | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| TRR ECR | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| ECR TRR | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 659015 | |
| ECR TRR | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| TRR ECR | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 659013 | |
| ECR TRR | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 659016 |