E(SPL)M8-HLH
Gene Ontology Biological Process
- Notch signaling pathway [NAS]
- cell fate commitment [NAS]
- compound eye development [IMP, TAS]
- dendrite morphogenesis [IMP]
- ectoderm development [TAS]
- epidermal growth factor receptor signaling pathway [NAS]
- imaginal disc-derived wing margin morphogenesis [NAS]
- mesoderm development [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISS, NAS]
- neuron development [IMP]
- peripheral nervous system development [TAS]
- regulation of R8 cell spacing in compound eye [NAS]
- sensory organ precursor cell fate determination [IMP]
Gene Ontology Molecular Function- DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
GRO
Gene Ontology Biological Process
- actin filament organization [IMP]
- dendrite morphogenesis [IMP]
- lateral inhibition [IMP]
- negative regulation of Wnt signaling pathway [NAS]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of smoothened signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IPI, TAS]
- negative regulation of transcription, DNA-templated [IDA, IGI, NAS, TAS]
- nervous system development [IMP, NAS]
- regulation of transcription, DNA-templated [IGI, IMP]
- sex determination, primary response to X:A ratio [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleus [NAS, TAS]
- protein complex [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability.
Proneural bHLH activators are expressed in all neuroectodermal regions prefiguring events of central and peripheral neurogenesis. Drosophila Sc is a prototypical proneural activator that heterodimerizes with the E-protein Daughterless (Da) and is antagonized by, among others, the E(spl) repressors. We determined parameters that regulate Sc stability in Drosophila S2 cells. We found that Sc is a very labile phosphoprotein and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| E(SPL)M8-HLH GRO | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| GRO E(SPL)M8-HLH | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| E(SPL)M8-HLH GRO | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - |