PREY

SHC4

RaLP, SHCD, UNQ6438/PRO21364
SHC (Src homology 2 domain containing) family, member 4
GO Process (1)
GO Function (3)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The ShcD phosphotyrosine adaptor subverts canonical EGF receptor trafficking.

Wills MKB, Lau HR, Jones N

Shc family signalling adaptors connect activated transmembrane receptors to proximal effectors, and most also contain a sequence involved in clathrin-mediated receptor endocytosis. Notably, this AP2 adaptin-binding motif (AD) is absent from the ShcD (also known as Shc4) homolog, which also uniquely promotes ligand-independent phosphorylation of the epidermal growth factor receptor (EGFR). We now report that cultured cells expressing ShcD exhibit ... [more]

J. Cell. Sci. Sep. 01, 2017; 130(17);2808-2820 [Pubmed: 28724758]

Throughput

  • Low Throughput

Curated By

  • BioGRID