BAIT

AAR2

C20orf4, CGI-23
AAR2 splicing factor homolog (S. cerevisiae)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

BAG2

BAG-2, dJ417I1.2, RP3-496N17.2
BCL2-associated athanogene 2
Alzheimer's Disease Project
GO Process (2)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

Malinova A, Cvackova Z, Mateju D, Horejsi Z, Abeza C, Vandermoere F, Bertrand E, Stanek D, Verheggen C

Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the HSP90/R2TP ... [more]

J. Cell Biol. Dec. 05, 2016; 216(6);1579-1596 [Pubmed: 28515276]

Throughput

  • High Throughput

Curated By

  • BioGRID