BAIT

AGO2

EIF2C2, Q10
argonaute RISC catalytic component 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

saRNA-guided Ago2 targets the RITA complex to promoters to stimulate transcription.

Portnoy V, Lin SH, Li KH, Burlingame A, Hu ZH, Li H, Li LC

Small activating RNAs (saRNAs) targeting specific promoter regions are able to stimulate gene expression at the transcriptional level, a phenomenon known as RNA activation (RNAa). It is known that RNAa depends on Ago2 and is associated with epigenetic changes at the target promoters. However, the precise molecular mechanism of RNAa remains elusive. Using human CDKN1A (p21) as a model gene, ... [more]

Cell Res. Mar. 01, 2016; 26(3);320-35 [Pubmed: 26902284]

Throughput

  • Low Throughput

Curated By

  • BioGRID