An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence.

Burla R, Carcuro M, Torre ML, Fratini F, Crescenzi M, D'Apice MR, Spitalieri P, Raffa GD, Astrologo L, Lattanzi G, Cundari E, Raimondo D, Biroccio A, Gatti M, Saggio I

AKTIP is a shelterin-interacting protein required for replication of telomeric DNA. Here, we show that AKTIP biochemically interacts with A- and B-type lamins and affects lamin A, but not lamin C or B, expression. In interphase cells, AKTIP localizes at the nuclear rim and in discrete regions of the nucleoplasm just like lamins. Double immunostaining revealed that AKTIP partially co-localizes ... [more]

Open Biol Dec. 01, 2015; 6(8); [Pubmed: 27512140]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

AKTIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]small conjugating protein transferase activity [IBA, NAS]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]

Gene Ontology Cellular Component

TKT

Gene Ontology Biological Process

Gene Ontology Molecular Function

cofactor binding [IDA]protein homodimerization activity [IDA]transketolase activity [EXP, IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence.

Throughput

Curated By

protein binding [IPI]
small conjugating protein transferase activity [IBA, NAS]
ubiquitin protein ligase activity [IBA]
ubiquitin protein ligase binding [IBA]

cofactor binding [IDA]
protein homodimerization activity [IDA]
transketolase activity [EXP, IDA]