CUL5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EPHA2
Gene Ontology Biological Process
- activation of Rac GTPase activity [IMP]
- bone remodeling [ISS]
- branching involved in mammary gland duct morphogenesis [ISS]
- cell chemotaxis [IMP]
- cell migration [IMP]
- ephrin receptor signaling pathway [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- keratinocyte differentiation [IMP]
- lens fiber cell morphogenesis [ISS]
- mammary gland epithelial cell proliferation [ISS]
- multicellular organismal development [TAS]
- negative regulation of protein kinase B signaling [IDA]
- osteoblast differentiation [ISS]
- osteoclast differentiation [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- protein kinase B signaling [IDA]
- regulation of ERK1 and ERK2 cascade [IMP]
- regulation of angiogenesis [ISS]
- regulation of blood vessel endothelial cell migration [ISS]
- regulation of cell adhesion mediated by integrin [IDA]
- regulation of lamellipodium assembly [IMP]
- response to growth factor [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SOCS2 Binds to and Regulates EphA2 through Multiple Mechanisms.
Suppressors of cytokine signaling (SOCS) proteins inhibit signaling by serving as substrate receptors for the Cullin5-RING E3 ubiquitin ligase (CRL5) and through a variety of CRL5-independent mechanisms. CRL5, SOCS2 and SOCS6 are implicated in suppressing transformation of epithelial cells. We identified cell proteins that interact with SOCS2 and SOCS6 using two parallel proteomics techniques: BioID and Flag affinity purification mass ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID