BAIT
WDTC1
Gm695, adipose, adp, RP23-32C12.5
WD and tetratricopeptide repeats 1
GO Process (9)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- cellular chemical homeostasis [IMP]
- cellular response to insulin stimulus [IMP]
- glucose metabolic process [IMP]
- in utero embryonic development [IMP]
- multicellular organism growth [IMP]
- negative regulation of catalytic activity [IDA]
- negative regulation of fatty acid biosynthetic process [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell size [IMP]
Gene Ontology Molecular Function
Mus musculus
PREY
RAB14
0610030G24Rik, 2810475J17Rik, A830021G03Rik, AI314285, AI649155, D030017L14Rik, RP23-186B18.6
RAB14, member RAS oncogene family
GO Process (11)
GO Function (6)
GO Component (22)
Gene Ontology Biological Process
- GTP catabolic process [IBA, ISO]
- Golgi to endosome transport [IMP]
- Rab protein signal transduction [IBA]
- apical protein localization [ISO]
- body fluid secretion [ISO]
- embryo development [IMP]
- endocytic recycling [ISO]
- fibroblast growth factor receptor signaling pathway [IMP]
- intracellular protein transport [IBA]
- phagolysosome assembly involved in apoptotic cell clearance [IBA]
- regulation of protein localization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IBA]
- Golgi stack [ISO]
- alveolar lamellar body [ISO]
- apical plasma membrane [ISO]
- cell [IMP]
- cytoplasm [IMP]
- cytosol [ISO]
- early endosome [IBA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular [IMP, ISO]
- intracellular membrane-bounded organelle [ISO]
- late endosome [ISO]
- lysosomal membrane [ISO]
- lysosome [ISO]
- nuclear outer membrane-endoplasmic reticulum membrane network [ISO]
- perinuclear region of cytoplasm [ISO]
- phagocytic vesicle [IBA, ISO]
- plasma membrane [ISO]
- recycling endosome [ISO]
- rough endoplasmic reticulum [ISO]
- trans-Golgi network transport vesicle [ISO]
- transport vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The antiobesity factor WDTC1 suppresses adipogenesis via the CRL4WDTC1 E3 ligase.
WDTC1/Adp encodes an evolutionarily conserved suppressor of lipid accumulation. While reduced WDTC1 expression is associated with obesity in mice and humans, its cellular function is unknown. Here, we demonstrate that WDTC1 is a component of a DDB1-CUL4-ROC1 (CRL4) E3 ligase. Using 3T3-L1 cell culture model of adipogenesis, we show that disrupting the interaction between WDTC1 and DDB1 leads to a ... [more]
EMBO Rep. May. 01, 2016; 17(5);638-47 [Pubmed: 27113764]
Throughput
- High Throughput
Curated By
- BioGRID