BAIT
WDTC1
Gm695, adipose, adp, RP23-32C12.5
WD and tetratricopeptide repeats 1
GO Process (9)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- cellular chemical homeostasis [IMP]
- cellular response to insulin stimulus [IMP]
- glucose metabolic process [IMP]
- in utero embryonic development [IMP]
- multicellular organism growth [IMP]
- negative regulation of catalytic activity [IDA]
- negative regulation of fatty acid biosynthetic process [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell size [IMP]
Gene Ontology Molecular Function
Mus musculus
PREY
MDM2
1700007J15Rik, AA415488, Mdm-2
transformed mouse 3T3 cell double minute 2
GO Process (24)
GO Function (9)
GO Component (9)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- establishment of protein localization [ISO]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of protein processing [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- peptidyl-lysine modification [ISO]
- positive regulation of cell cycle [IGI]
- positive regulation of gene expression [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein export from nucleus [ISO]
- protein catabolic process [IDA]
- protein complex assembly [ISO]
- protein destabilization [ISO]
- protein localization to nucleus [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of protein catabolic process [ISO]
- traversing start control point of mitotic cell cycle [IDA]
Gene Ontology Molecular Function
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The antiobesity factor WDTC1 suppresses adipogenesis via the CRL4WDTC1 E3 ligase.
WDTC1/Adp encodes an evolutionarily conserved suppressor of lipid accumulation. While reduced WDTC1 expression is associated with obesity in mice and humans, its cellular function is unknown. Here, we demonstrate that WDTC1 is a component of a DDB1-CUL4-ROC1 (CRL4) E3 ligase. Using 3T3-L1 cell culture model of adipogenesis, we show that disrupting the interaction between WDTC1 and DDB1 leads to a ... [more]
EMBO Rep. May. 01, 2016; 17(5);638-47 [Pubmed: 27113764]
Throughput
- High Throughput
Curated By
- BioGRID