An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex.

Khan MH, Salomaa SI, Jacquemet G, Butt U, Miihkinen M, Deguchi T, Kremneva E, Lappalainen P, Humphries MJ, Pouwels J

Sharpin, a multifunctional adaptor protein, regulates several signalling pathways. For example, Sharpin enhances signal-induced NF-ÎºB signalling as part of the linear ubiquitin assembly complex (LUBAC) and inhibits integrins, the T cell receptor, caspase 1 and PTEN. However, despite recent insights into Sharpin and LUBAC function, a systematic approach to identify the signalling pathways regulated by Sharpin has not been reported. ... [more]

J. Cell. Sci. Sep. 15, 2017; 130(18);3094-3107 [Pubmed: 28775156]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

SHARPIN

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

NEK7