Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.

Hoffmeister H, Fuchs A, Erdel F, Pinz S, Groebner-Ferreira R, Bruckmann A, Deutzmann R, Schwartz U, Maldonado R, Huber C, Dendorfer AS, Rippe K, Laengst G

CHD3 and CHD4 (Chromodomain Helicase DNA binding protein), two highly similar representatives of the Mi-2 subfamily of SF2 helicases, are coexpressed in many cell lines and tissues and have been reported to act as the motor subunit of the NuRD complex (nucleosome remodeling and deacetylase activities). Besides CHD proteins, NuRD contains several repressors like HDAC1/2, MTA2/3 and MBD2/3, arguing for ... [more]

Nucleic Acids Res. Oct. 13, 2017; 45(18);10534-10554 [Pubmed: 28977666]

Throughput

  • High Throughput

Additional Notes

  • nuclear extract

Curated By

  • BioGRID