BAIT

ALP6

SPBC902.01c, SPBC428.20c
gamma tubulin complex Spc98/GCP3 subunit Alp6
Schizosaccharomyces pombe (972h)
PREY

ALP16

SPCC4G3.19
gamma tubulin complex subunit Alp16
Schizosaccharomyces pombe (972h)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function.

Venkatram S, Tasto JJ, Feoktistova A, Jennings JL, Link AJ, Gould KL

The gamma-tubulin complex, via its ability to organize microtubules, is critical for accurate chromosome segregation and cytokinesis in the fission yeast, Schizosaccharomyces pombe. To better understand its roles, we have purified the S. pombe gamma-tubulin complex. Mass spectrometric analyses of the purified complex revealed known components and identified two novel proteins (i.e., Mbo1p and Gfh1p) with homology to gamma-tubulin-associated proteins ... [more]

Mol. Biol. Cell May. 01, 2004; 15(5);2287-301 [Pubmed: 15004232]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ALP6 ALP16
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
428817
ALP16 ALP6
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
247583

Curated By

  • BioGRID