BAIT

TNF

DIF, TNF-alpha, TNFA, TNFSF2, DADB-70P7.1
tumor necrosis factor
GO Process (75)
GO Function (6)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens
PREY

CHUK

IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
conserved helix-loop-helix ubiquitous kinase
GO Process (33)
GO Function (6)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SPATA2 links CYLD to the TNF-α receptor signaling complex and modulates the receptor signaling outcomes.

Wagner SA, Satpathy S, Beli P, Choudhary C

TNF-α is a key regulator of innate immune and proinflammatory responses. However, the composition of the TNF-α receptor-associated signaling complexes (TNF-RSC) and the architecture of the downstream signaling networks are incompletely understood. We employed quantitative mass spectrometry to demonstrate that TNF-α stimulation induces widespread protein phosphorylation and that the scope of phosphorylation expands in a temporal manner. TNF-α stimulation also ... [more]

EMBO J. Dec. 01, 2015; 35(17);1868-84 [Pubmed: 27307491]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TNF CHUK
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
TNF CHUK
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
TNF CHUK
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
940951

Curated By

  • BioGRID