BAIT

ARIH1

ARI, HARI, HHARI, UBCH7BP, HUSSY-27
ariadne RBR E3 ubiquitin protein ligase 1
UPS Project
Homo sapiens
PREY

FARSB

FARSLB, FRSB, PheHB, PheRS, HSPC173
phenylalanyl-tRNA synthetase, beta subunit
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.

Scott DC, Rhee DY, Duda DM, Kelsall IR, Olszewski JL, Paulo JA, de Jong A, Ovaa H, Alpi AF, Harper JW, Schulman BA

Hundreds of human cullin-RING E3 ligases (CRLs) modify thousands of proteins with ubiquitin (UB) to achieve vast regulation. Current dogma posits that CRLs first catalyze UB transfer from an E2 to their client substrates and subsequent polyubiquitylation from various linkage-specific E2s. We report an alternative E3-E3 tagging cascade: many cellular NEDD8-modified CRLs associate with a mechanistically distinct thioester-forming RBR-type E3, ARIH1, ... [more]

Cell Aug. 25, 2016; 166(5);1198-1214.e24 [Pubmed: 27565346]

Throughput

  • High Throughput

Curated By

  • BioGRID