Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.

Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP

High-throughput binary protein interaction mapping is continuing to extend our understanding of cellular function and disease mechanisms. However, we remain one or two orders of magnitude away from a complete interaction map for humans and other major model organisms. Completion will require screening at substantially larger scales with many complementary assays, requiring further efficiency gains in proteome-scale interaction mapping. Here, ... [more]

Mol. Syst. Biol. Apr. 22, 2016; 12(4);863 [Pubmed: 27107012]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

EAF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II regulatory region sequence-specific DNA binding [IDA]RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]protein binding [IPI]

Gene Ontology Cellular Component

NECAB2

Gene Ontology Molecular Function

protein binding [IPI]

Two-hybrid

Publication

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.

Throughput

Curated By

RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
protein binding [IPI]