BAIT

BRIP1

BACH1, FANCJ, OF
BRCA1 interacting protein C-terminal helicase 1
Fanconi Anemia Project
Homo sapiens
PREY

FAM96B

MIP18, CGI-128
family with sequence similarity 96, member B
GO Process (3)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

IOP1 protein is an external component of the human cytosolic iron-sulfur cluster assembly (CIA) machinery and functions in the MMS19 protein-dependent CIA pathway.

Seki M, Takeda Y, Iwai K, Tanaka K

The emerging link between iron metabolism and genome integrity is increasingly clear. Recent studies have revealed that MMS19 and cytosolic iron-sulfur cluster assembly (CIA) factors form a complex and have central roles in CIA pathway. However, the composition of the CIA complex, particularly the involvement of the Fe-S protein IOP1, is still unclear. The roles of each component are also ... [more]

J. Biol. Chem. Jun. 07, 2013; 288(23);16680-9 [Pubmed: 23585563]

Throughput

  • Low Throughput

Curated By

  • BioGRID