An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N

Parkin-mediated mitophagy is a quality control pathway that selectively removes damaged mitochondria via the autophagic machinery. Autophagic receptors, which interact with ubiquitin and Atg8 family proteins, contribute to the recognition of damaged mitochondria by autophagosomes. NDP52, an autophagy receptor, is required for autophagic engulfment of damaged mitochondria during mitochondrial uncoupler treatment. The N-terminal SKICH domain and C-terminal zinc finger motif ... [more]

EMBO Rep. Oct. 11, 2018; (); [Pubmed: 30309841]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CALCOCO2 SPECC1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zellner S (2021)	High	-	BioGRID	3306655

Curated By

BioGRID

Interaction Summary

CALCOCO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

SPECC1