Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP45 and Spindly are part of the same complex implicated in cell migration.

Conte C, Griffis ER, Hickson I, Perez-Oliva AB

Ubiquitylation is a protein modification implicated in several cellular processes. This process is reversible by the action of deubiquinating enzymes (DUBs). USP45 is a ubiquitin specific protease about which little is known, aside from roles in DNA damage repair and differentiation of the vertebrate retina. Here, by using mass spectrometry we have identified Spindly as a new target of USP45. ... [more]

Sci Rep Sep. 26, 2018; 8(1);14375 [Pubmed: 30258100]

Throughput

  • High Throughput

Curated By

  • BioGRID