Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Autophagy acts through TRAF3 and RELB to regulate gene expression via antagonism of SMAD proteins.

Newman AC, Kemp AJ, Drabsch Y, Behrends C, Wilkinson S

Macroautophagy can regulate cell signalling and tumorigenesis via elusive molecular mechanisms. We establish a RAS mutant cancer cell model where the autophagy gene ATG5 is dispensable in A549 cells in vitro, yet promotes tumorigenesis in mice. ATG5 represses transcriptional activation by the TGFβ-SMAD gene regulatory pathway. However, autophagy does not terminate cytosolic signal transduction by TGFβ. Instead, we use proteomics ... [more]

Nat Commun Dec. 16, 2016; 8(1);1537 [Pubmed: 29146913]

Throughput

  • High Throughput

Curated By

  • BioGRID