BAIT

GPSM1

AGS3, RP13-270L15.1
G-protein signaling modulator 1
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

MARCKSL1

AL022768, AW215397, AW536807, D4Bc1, F52, MacMARCKS, Macs2, Macs3, Mlp, Mrp, RP23-209C6.6
MARCKS-like 1
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Mus musculus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction of activator of G-protein signaling 3 (AGS3) with LKB1, a serine/threonine kinase involved in cell polarity and cell cycle progression: phosphorylation of the G-protein regulatory (GPR) motif as a regulatory mechanism for the interaction of GPR motifs with Gi alpha.

Blumer JB, Bernard ML, Peterson YK, Nezu J, Chung P, Dunican DJ, Knoblich JA, Lanier SM

Activator of G-protein signaling 3 (AGS3) has a modular domain structure consisting of seven tetratricopeptide repeats (TPRs) and four G-protein regulatory (GPR) motifs. Each GPR motif binds to the alpha subunit of Gi/Go (Gialpha > Goalpha) stabilizing the GDP-bound conformation of Galpha and apparently competing with Gbetagamma for GalphaGDP binding. As an initial approach to identify regulatory mechanisms for AGS3-G-protein ... [more]

J. Biol. Chem. Jun. 27, 2003; 278(26);23217-20 [Pubmed: 12719437]

Throughput

  • High Throughput

Curated By

  • BioGRID