Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H, Kimura M, Kanda E, Ishihama A

RNA polymerase II (pol II) purified from the fission yeast Schizosaccharomyces pombe was previously reported to be associated with the general transcription factor TFIIF and the C-terminal domain phosphatase Fcp1, as well as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which has recently been implicated in transcriptional activation in human cells. Here, we provide evidence that the Rpb7 subunit of pol II interacts with ... [more]

FEBS Lett. Jan. 03, 2005; 579(1);48-52 [Pubmed: 15620689]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPB7 GPD3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-5.9621	BioGRID	761371

Curated By

BioGRID

Interaction Summary

RPB7

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-directed RNA polymerase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

GPD3