An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Molecular analysis of kinetochore architecture in fission yeast.

Liu X, McLeod I, Anderson S, Yates JR, He X

Kinetochore composition and structure are critical for understanding how kinetochores of different types perform similar functions in chromosome segregation. We used affinity purification to investigate the kinetochore composition and assembly in Schizosaccharomyces pombe. We identified a conserved DASH complex that functions to ensure precise chromosome segregation. Unlike DASH in budding yeast that is localized onto kinetochores throughout the cell cycle, ... [more]

EMBO J. Aug. 17, 2005; 24(16);2919-30 [Pubmed: 16079914]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ASK1 DAD1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu X (2005)	Low	-	BioGRID	-
DAD1 ASK1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Roguev A (2008)	High	-2.8404	BioGRID	524447

Curated By

BioGRID

Interaction Summary

DAD1

Gene Ontology Biological Process

Gene Ontology Cellular Component

ASK1